Coding
Part:BBa_K1152003:Design
Designed by: Tania Christiansen Group: iGEM13_Heidelberg (2013-09-20)
TycC6 Module from Tyrocidine
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 48
Illegal SapI.rc site found at 1165
Illegal SapI.rc site found at 1402
Design Notes
This is just the coding sequence. We sequenced with VR and VF2 primer, one silent mutation was found at position silent mutations: 4595 GTA (Val) to GTG (Val) (http://2013.igem.org/File:Heidelberg_RFC10_TycC6Seq.zip and http://2013.igem.org/Team:Heidelberg/Tyrocidine_week20_biobrickmod#Sequencing). You can use it to combine it with other modules of e.g. the Tyrocidine cluster for the construction of a new non ribosomal-peptide. http://2013.igem.org/Team:Heidelberg/Parts
Source
Tyrocidine Gene Cluster of Brevibacillus Parabrevis. Genebank File taken from http://www.ncbi.nlm.nih.gov/nuccore/af004835